Open Resources

Adsorption Protocol: The initial stage of infection, termed the external stage, involves the adsorption of the phage to a receptor (polysaccharide or extra-membrane protein). This adsorption traps the phages on the surface of cells, leading to a decrease in the quantity of free phage in the medium. This phenomenon is measured in the assay described.

Phage DNA Extraction: A protocol to obtain high quality phage DNA.

Burst Protocol: Here we use a simplified method to assess the latency time and size of progeny released by a single cell (burst size).

High titer phages protocol: Here you will find a method that will allow you to obtain high concentration of phages.

Gene inactivation or deletion in vibrios: This method will help you plan the required steps for the inactivation or deletion of genes in vibrios.

Phage isolation protocol: This method will allow you to obtain pure, high quality phages from streaking.

Vibrio conjugation protocol: This method will allow you to obtain bacteria with plasmids of interest.